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Chemical composition,antioxidant and antimicrobial activities of the edible medicinal Ononis natrix growing wild in Tunisia
Authors:Baya Mhamdi  Feten Abbassi  Chedly Abdelly
Institution:1. Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba, BP: 382, Béja 9000, Tunisie;2. Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj-Cédria, BP 901, 2050 Hammam-Lif, Tunisia;3. Ecole Supérieure des Sciences et Techniques de la Santé de Monastir, Université de Monastir, Avenue Avicenne B.P. 128, 5060 Monastir, Tunisie;4. Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj-Cédria, BP 901, 2050 Hammam-Lif, Tunisia
Abstract:Results showed that leaf methanolic extract of Ononis natrix has important total phenol (51 mg GAE/g DW) and flavonoid (14.76 CE/g DW) contents. The chemical composition of O. natrix leaf revealed the presence of quercitine (24.5%), amentoflavone (14.1%), flavones (11.3%) and kaempferol (10.5%). The leaf extract showed a high total antioxidant activity with 60.94 mg of GAE/g DW, displayed a high 2,2-diphenyl-1-picrylhydrazyl scavenging ability with low IC50 value (29 μg/mL) and a great reducing power (EC50 = 100 μg/mL). O. natrix leaf extract exhibited a significant broad spectrum activity against all tested microorganisms with bacterial inhibition zone sizes ranging from 8.5 to 17 mm in diameter.
Keywords:Ononis natrix  phenolic identification  antimicrobial activity
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