Ethanolic extract of Ferula gummosa is cytotoxic against cancer cells by inducing apoptosis and cell cycle arrest |
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| Authors: | Hoda Gudarzi Saeed Irian Amir Amanzadeh Hirsa Mostafapour Kandelous Keyhan Azadmanesh |
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| Institution: | 1. Department of Biology, Faculty of Science, Kharazmi University, Tehran, Iran;2. Department of Cellular and Molecular Biology, Faculty of Science, Kharazmi University, Tehran, Iran;3. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran;4. Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran;5. Department of Virology, Pasteur Institute of Iran, Tehran, Iran |
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| Abstract: | Ferula gummosa Boiss. has medicinal applications in treating a wide range of diseases including cancer. The objective of this study was to evaluate the antiproliferative activities of the seed and gum extracts of F. gummosa as well as to study the effect of the potent extract on the induction of apoptosis and cell cycle arrest. Our results demonstrated that the ethanolic extract had the lowest IC50 value at 72 h (0.001 ± 1.2 mg/mL) in BHY cells. Moreover, flowcytometry and annexin-V analysis revealed that the ethanolic extract induced apoptosis and cell-cycle arrest in BHY cells at G1/S phase. In addition, colorimetric methods exhibited the highest amount of total phenolics and flavonoids in the aqueous and gum extracts (0.12 ± 0.037, 0.01 ± 2.51 mg/g of dry powder). Generally, the results obtained indicate that F. gummosa ethanol extract may contain effective compounds which can be used as a chemotherapeutic agent. |
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| Keywords: | Ferula gummosa cancer apoptosis MTT assay flowcytometry |
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