Exploration of genetic diversity among medicinally important genus Epimedium species based on genomic and EST-SSR marker |
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| Authors: | Zubaida Yousaf Weiming Hu Yanjun Zhang Shaohua Zeng Ying Wang |
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| Institution: | 1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Gaungzhou 510650, PR China;2. Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, the Chinese Academy of Sciences, Wuhan, Hubei, PR China;3. Department of Botany, Lahore College for Women University, Jail Road Lahore, Pakistan;4. Department of Botany, Lahore College for Women University, Jail Road Lahore, Pakistan |
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| Abstract: | Epimedium species has gained prime importance due to their medicinal and economic values. Therefore, in this study, 26 genomic SSR and 10 EST-SSR markers were developed for 13 medicinal species of the Epimedium genus and one out-group species Vancouveria hexandra W. J. Hooker to explore the existing genetic diversity. A total of 100 alleles by genomic SSR and 65 by EST-SSR were detected. The genomic SSR markers were presented between 2–7 alleles per locus. The observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.00 to 4.5 and 0.0254 to 2.8108, respectively. Similarly, for EST-SSR, these values were ranged from 3.00 to 4.00 and 1.9650 to 2.7142. The number of alleles for EST-SSR markers ranged from 3 to 10 with an average of 3.51 per loci. It has been concluded that medicinally important species of the genus Epimedium possesses lower intraspecific genetic variation. |
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| Keywords: | Epimedium genomic SSR/EST SSR markers genetic diversity Systematics |
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