Production and extraction of astaxanthin from Phaffia rhodozyma and its biological effect on alcohol-induced renal hypoxia in Carassius auratus |
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Authors: | Alessio Alesci Andrea Salvo Eugenia Rita Lauriano Teresa Gervasi Deborah Palombieri Maurizio Bruno |
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Affiliation: | 1. Department of Environmental Sciences, Territorial, Food and Health Security (S.A.S.T.A.S.), University of Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy;2. Department of Biological and Environmental Sciences, University of Messina, Viale Stagno d'Alcontres 31, 98166 Messina, Italy;3. Department of Biological Technology, Chemical and Pharmaceutical Science, University of Palermo, Viale delle Scienze, Parco d'Orleans II, 90128 Palermo, Italy |
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Abstract: | The effect of astaxanthin (3,3′-dihydroxy-s-carotene-4,4′-dione) on alcohol-induced morphological changes in Carassius auratus, as an experimental model, was determined. The yeast Phaffia rhodozyma was used as a source of astaxanthin. The animals were divided into three groups for 30 days: one group was treated with ethanol at a dose of 1.5% mixed in water, the second one with EtOH 1.5% and food enriched with astaxanthin from P. rhodozyma, and the third was a control group. After a sufficient experimental period, the samples were processed using light microscopy and evaluated by histomorphological and histochemical staining, and the data were supported by immunohistochemical analysis, using a wide range of antibodies, such as calbindin, vimentin and alpha-smooth muscle actin. The results show that the alcoholic damage in the kidney led to hypoxia. In contrast, the group fed with astaxanthin from P. rhodozyma showed a normal morphological picture, with better glomeruli organisation and the presence of the area of filtration. Furthermore, the immunohistochemistry has confirmed these results. |
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Keywords: | Phaffia rhodozyma astaxanthin kidney Carassius auratus |
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