LSPR Biosensor Signal Enhancement Using Nanoparticle-Antibody Conjugates |
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Authors: | Hall W Paige Ngatia Salome N Van Duyne Richard P |
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Affiliation: | Department of Chemistry, Northwestern University, 2145 North Sheridan Road, Evanston, Illinois 60208, United States. |
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Abstract: | A method to amplify the wavelength shift observed from localized surface plasmon resonance (LSPR) bioassays is developed using gold nanoparticle-labeled antibodies. The technique, which involves detecting surface-bound analytes using gold nanoparticle conjugated antibodies, provides a way to enhance LSPR shifts for more sensitive detection of low-concentration analytes. Using the biotin and antibiotin binding pair as a model, we demonstrate up to a 400% amplification of the shift upon antibody binding to analyte. In addition, the antibody-nanoparticle conjugate improves the observed binding constant by 2 orders of magnitude, and the limit of detection by nearly 3 orders of magnitude. This amplification strategy provides a way to improve the sensitivity of plasmon-based bioassays, paving the way for single molecule-based detection and clinically relevant diagnostics. |
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