Some effective solvents for rapid and reliable characterization of phenylthiohydantoin-amino acids |
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Authors: | R L Munier A M Drapier |
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Institution: | (1) C.N.R.S., Institut Pasteur, 28 rue du Docteur Roux, F-75015 Paris, France |
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Abstract: | Summary One-dimensional chromatography with internal standards permits reliable identification of the phenylthiohydantoins from all the common amino acids with the following TLC systems: silica gel — chloroform /n-butyl acetate (90 10), di-isopropylether/ethanol (95 5), dichloromethane/ethanol/acetic acid (90 8 2) ortrans-dichloroethylene/ethanol/acetic acid (88 10 2); and cellulose with 25% formic acid — heptane/isobutanol/75% fromic acid (40 30 9) or silica gel — chloroform / ethanol/acetic acid/water (50 47 0.5 2.5).Abbreviations: PTH=phenylthiohydantoin, TLC=thin-layer chromatography, HPTLC=high-performance TLC; other abbreviations: see end of text. Proportions in solvent mixtures are v/v except where otherwise indicated. |
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Keywords: | Effective solvents Phenylthiohydatoin-amino acids Reliable characterization Thin-layer chromatography Silica gel |
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