Some effective solvents for rapid and reliable characterization of phenylthiohydantoin-amino acids

Summary One-dimensional chromatography with internal standards permits reliable identification of the phenylthiohydantoins from all the common amino acids with the following TLC systems: silica gel — chloroform/n-butyl acetate (9010), di-isopropylether/ethanol (955), dichloromethane/ethanol/acetic acid (9082) ortrans-dichloroethylene/ethanol/acetic acid (88102); and cellulose with 25% formic acid — heptane/isobutanol/75% fromic acid (40309) or silica gel — chloroform/ ethanol/acetic acid/water (50470.52.5).Abbreviations: PTH=phenylthiohydantoin, TLC=thin-layer chromatography, HPTLC=high-performance TLC; other abbreviations: see end of text. Proportions in solvent mixtures are v/v except where otherwise indicated.