Determination of average degree of polymerisation and distribution of oligosaccharides in a partially acid-hydrolysed homopolysaccharide: a comparison of four experimental methods applied to mannuronan |
| |
Authors: | Campa Cristiana Oust Astrid Skjåk-Braek Gudmund Paulsen Berit Smestad Paoletti Sergio Christensen Bjørn E Ballance Simon |
| |
Institution: | Department of Biochemistry, Biophysics and Macromolecular Chemistry University of Trieste, 1-34127 Trieste, Italy. |
| |
Abstract: | The average degree of polymerisation (DP) and distribution of oligosaccharides in partially acid hydrolysed mannuronans were quantitatively evaluated by 1H NMR, electrospray ionisation mass spectrometry (ESI-MS), micellar electrokinetic capillary chromatography with UV detection (MEKC-UV), and high-pressure anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Our investigation shows that 1H NMR, MEKC-UV and, in particular, HPAEC-PAD can be used as quantitative tools to aid the investigation of polysaccharide structure, function and synthesis. For the latter two techniques, especially, this represents a significant new development as it enables calculation of the quantity of individual oligomers of nominal DP by direct analysis of a defined oligomer mixture. Appropriate statistical averages of number and weight distributions were also calculated and found to fit very well to predicted Kuhn distributions that assume random depolymerisation. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|