Determination of Catechin in Aqueous Solution by Chemiluminescence Method |
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Authors: | Jung?Min?Lee Mohammad?Mainul?Karim Email author" target="_blank">Sang?Hak?LeeEmail author |
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Institution: | (1) Department of Chemistry, Kyungpook National University, Taegu, 702-701, Korea |
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Abstract: | A method to determine catechin in aqueous solution by measuring chemiluminescence intensities using a stopped flow system
has been studied. The lucigenin-hydrogen peroxide chemiluminescence reaction was chosen for the determination of catechin.
Fe(II) ion was added to the chemiluminescence system to increase the sensitivity. The chemiluminescence intensity from the
lucigenin system was increased by the addition of catechin. Effects of flow rates of reagent and sample and concentrations
of lucigenin, hydrogen peroxide, Fe(II) ion and KOH were investigated. The calibration curve for catechin was linear over
the range from 1.0×10−6 to 1.0×10−3 M and the detection limit was 3.0×10−7 M under the optimal experimental conditions. |
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Keywords: | Catechin chemiluminescence |
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