Study of catalase immobilized on a silicate matrix for non-aqueous biocatalysis |
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| Authors: | Elena Horozova Nina Dimcheva |
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| Institution: | (1) Department of Physical Chemistry, Plovdiv University, 24, Tsar Assen st., 4000 Plovdiv, Bulgaria |
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| Abstract: | Catalytic activity of catalase (CAT) immobilized on a modified silicate matrix to mediate decomposition of meta-chloroperoxibenzoic
acid (3-CPBA) in acetonitrile has been investigated by means of quantitative UV-spectrophotometry. Under the selected experimental
conditions, the kinetic parameters: the apparent Michaelis constat (K
M
), the apparent maximum rate of enzymatic reaction (V
max
app
), the first order specific rate constants (k
sp
), the energy of activation (E
a
) and the pre-exponential factor of the Arrhenius equation (Z0) were calculated. Conclusions regarding the rate-limiting step of the overall catalytic process were drawn from the calculated
values of the Gibbs energy of activation ΔG*, the enthalpy of activation ΔH*, and the entropy of activation ΔS*. |
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| Keywords: | Non-aqueous biocatalysis immobilized catalase silicate carrier meta-Cloroperoxibenzoic acid |
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