In this work, a novel and sensitive analytical methodology for mercury preconcentration and determination in different water samples using ionic liquid was developed. The use of room temperature ionic liquid‘s biphasic systems as an alternative to conventional solvents offers several advantages including safety and high capacity to extract Hg(II) and other elements with high recoveries. ?onic liquid in combination with porphyrin complexing reagent was successfully applied in this study for the extraction and preconcentration of Hg(II). Likewise, a fast and quantitative back extraction of the analyte from room temperature ionic liquid phase into aqueous phase was possible, allowing its further determination by spectrofluorimetry. The preconcentration method allowed mercury determination in tap, river and mineral water samples at trace levels with high accuracy and reproducibility.