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Amperometric biosensor for hydrogen peroxide based on the co-electrodeposition of horseradish peroxidase and methylene blue on an ITO electrode modified with an anodic aluminum oxide template |
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| Authors: | Hui Yao Huan Liu Mei-jiao Sun Lei Gong |
| Institution: | 1. College of Applied Chemistry, Shenyang University of Chemical Technology, Shenyang, 110142, People’s Republic of China 2. College of Science, North University of China, Taiyuan, 030051, People’s Republic of China |
| Abstract: | We report on a nano-array sensor for hydrogen peroxide (H2O2) that is based on a nanoporous anodic aluminum oxide template. This was used as a matrix for the co-immobilization of horseradish peroxidase (HRP) and methylene blue (MB) on the surface of an indium tin oxide electrode. The immobilized HRP retained its natural activity and MB is capable of efficiently shuttle electrons between HRP and the electrode. The new electrode was characterized by SEM and electrochemical methods. It exhibits fast response, long-term stability, high sensitivity and good selectivity to H2O2. Under optimized conditions, it linearly responds to H2O2 in the concentration range from 1.0?μM to 26?mM, with a detection limit of 0.21?μM (at S/N?=?3). A nano-array biosensor for hydrogen peroxide (H2O2) based on the co-electrodeposition of horseradish peroxidase (HRP) and methylene blue (MB) into anodic aluminum oxide template was constructed. The immobilized HRP could maintain natural bioactivity and MB could efficiently shuttle electrons between HRP and the electrode. |
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