|
|||||
|
|
Electrochemical detection of in situ DNA damage induced by enzyme-catalyzed Fenton reaction. Part II in hydrophobic room temperature ionic liquid |
|
| Authors: | Yang Chen Huayu Xiong Xiuhua Zhang Shengfu Wang |
| Affiliation: | 1. Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, People’s Republic of China 2. Key Laboratory of Analytical Chemistry for Biology and Medicine (Wuhan University), Ministry of Education, Wuhan, People’s Republic of China |
| Abstract: | A hydrophobic room temperature ionic liquid, 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF6]), was applied as nonaqueous solvent for the generation of hydroxy radical (?OH) through glucose oxidase-catalyzed Fenton reaction. The enzyme catalyzes the oxidation of glucose, and the produced H2O2 further reacts with transition metal ions, generating hydroxyl radicals. They attacked DNA and led its damage. This was detected by square wave voltammetry (SWV) of the electroactive indicator Co(bpy) 3 3+ . It bound more strongly to intact DNA, and the SWV peak currents decreased at the potential of 0.064?V when DNA was damaged. The experimental results testified that the antioxidants, ascorbic acid, aloe-emodin and rutin, inhibited oxidative DNA damage by hydroxyl radicals. The method is promising for rapid, sensitive, and inexpensive detection of DNA damage. Schematic diagram for working principle of SWV detection of in situ DNA damage for DNA-GOx film. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |