基于蛋白质工程的人工金属酶——半合成金属酶在原子转移反应中的应用

近年来,随着生物技术的进步,基于蛋白质工程的人工金属酶?半合成金属酶的研究及应用取得了突飞猛进的发展.在原子转移反应中,半合成金属酶已经能够高效、高选择性地催化氧化、不对称氢化、碳-碳键偶联等多种反应.通过对蛋白质的突变和对人工辅酶的修饰与改进,可以实现对酶的功能、催化效率以及立体选择性等多方面的调控.通过对半合成金属酶的研究,能够更深入地理解二级配位环境,为设计和制备高效"绿色金属催化剂",以及为探索金属配合物与蛋白质的相互作用、发展无机金属药物提供崭新的途径.

Construction of artificial metalloenzyme toward atom transfer reactions by protein engineering

Along with the rapid development of biologic technology, constructing artificial metalloenzymes by rational combination of non-native metal cofactors and protein scaffolds attracts increasing attention. Towards the application in atom transfer reactions such as asymmetric oxidation, hydrogenation, hydrolysis, C-C bond formation, etc., artificial metalloenzymes exhibit high reactivity and selectivity. The function, efficiency, and stereoselectivity of enzymes can be adjusted through the mutation and modification of these artificial proteins. This approach can help understand secondary coordination sphere, and provide an access to design high efficiency ＂green metal catalyst＂, explore interactions between metal complex and protein, and develop new inorganic metal drugs.