| 药根碱与溶菌酶相互作用的光谱研究 |
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| 引用本文： | 李颖.药根碱与溶菌酶相互作用的光谱研究[J].光谱实验室,2010,27(5):1983-1987. |
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| 作者姓名： | 李颖 |
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| 作者单位： | 河海大学环境科学与工程学院,南京市西康路1号,210098 |
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| 基金项目： | 教育部重点实验室开放研究基金项目，河海大学自然科学基金项目 |
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| 摘 要： | 利用荧光光谱和红外光谱研究了药根碱与溶菌酶的结合反应。通过Stern-Volmer's方程分别计算了不同温度下反应的结合常数。FTIR光谱结果表明:药根碱与溶菌酶键合改变了溶菌酶原有的构象,使溶菌酶的α-螺旋和β-折叠的含量发生变化。根据Foerster非辐射能量转移理论,计算出药根碱与溶菌酶之间的结合距离为5.39nm。
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| 关 键 词： | 药根碱 溶菌酶 相互作用 |
Spectral Study on the Interaction Between Jatrorrhizine to Lysozyme |
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| LI Ying.Spectral Study on the Interaction Between Jatrorrhizine to Lysozyme[J].Chinese Journal of Spectroscopy Laboratory,2010,27(5):1983-1987. |
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| Authors: | LI Ying |
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| Institution: | LI Ying(College of Environmental Science and Engineering,Hehai University,Nanjing 210098,P.R.China) |
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| Abstract: | Optical properties of the jatrorrhizine-lysozyme complex were characterized by fluorescence and Fourier transform infrared（FT-IR）spectra.The binding constants at different temperature were calculated according to Stern-Volmer＇s equation.The protein conformation is altered with enhancement of α-helices and decrease of β-turn structure.The distance from the tryptophan residue（donor）to the bound drug（acceptor）in lysozyme was calculated as 5.39nm through Foerster non-radiation energy transform theory. |
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| Keywords: | Jatrorrhizine Lysozyme Interaction |
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