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Development and characterization of new polyclonal antibodies specific for three polychlorinated biphenyls |
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| Authors: | Han Yu Chen Hui Sheng Zhuang Chun Zhoua a College of Environmental Science Engineering Donghua University Shanghai China b School of Environmental Science Engineering Shanghai Jiaotong University Shanghai China c |
| Affiliation: | a College of Environmental Science and Engineering, DongHua University, Shanghai 201620, China;b School of Environmental Science and Engineering, Shanghai Jiaotong University, Shanghai 200240, China;c Department of Environment Engineering, Pingdingshan Institute of Technology, Henan 467000, China |
| Abstract: | Three polychlorinated biphenyls(PCBs)congeners and their corresponding haptens bearing four carbon length carboxylic groups that can be linked to a protein for raising antibodies were synthesized.The three resultant immunogens were fabricated and used to stimulate immune responses in rabbits to survey the characteristics of the haptens.Three of the resultant polyclonal antibodies(Pabs)were obtained.The antiserum exhibited relatively high antibody titres(1:32-64)in double agar diffusion.◎2008 Hui Sheng Zhuang.Published by Elsevier B.V.on behalf of Chinese Chemical Society.All rights reserved. |
| Keywords: | Immunoassays Polychlorinated biphenyls Haptens lmmunogens Polyclonal antibodies |
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