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Relationship between the structure of the protein globule and bioluminescence spectra of firefly luciferase |
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| Authors: | Ugarova N. N. Brovko L. Yu. |
| Affiliation: | (1) Department of Chemistry, M. V. Lomonosov Moscow State University, Leninskie Gory, 119899 Moscow, Russian Federation;(2) Department of Food Science, University of Guelph, Guelph, ON, N1G 2W1, Canada |
| Abstract: | Bioluminescence spectra for various native and mutant luciferases from fireflies and beetles were analyzed in the light of the known theoretical concepts on the influence of the microenvironment of the emitter on its emission spectra. The mechanism for the explanation of the nature of changing bioluminescence spectra for natural and artificial mutations of the amino acid residues in the protein globule of luciferases was proposed. |
| Keywords: | bioluminescence luciferase luciferin ATP fireflies bioluminescence spectra protein structure mutation of amino acid residues |
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