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An experimental investigation of three-dimensional particle aggregation using digital holographic microscopy
Institution:1. Computing Department, Faculty of Arts, Computing and Creative Industry, Sultan Idris Education University (UPSI), 35900 Tanjong Malim, Perak, Malaysia;2. Wolfson School of Mechanical and Manufacturing Engineering, Loughborough University, Loughborough, LE11 3TU, UK;1. Communication System Laboratory Sys׳Com, National Engineering School of Tunis, University Tunis El Manar, Tunisia;2. University of Monastir, Tunisia;3. UMR 6614 CORIA, Normandy University, Avenue de l׳Université, 76801 Saint-Etienne du Rouvray, France;1. Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan;2. Research Fellow of the Japan Society for the Promotion of Science, 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan;3. Department of Mechanical Engineering, Tokyo University of Science, Yamaguchi, Daigaku-dori 1-1-1, Sanyo-Onoda, Yamaguchi 756-0884, Japan;1. Department of Gastroenterology and Hepatology, Academic Medical Centre, Amsterdam, The Netherlands;2. Department of Gastroenterology, Dr. Horst Schmidt Klinik, Wiesbaden, Germany;3. Gastro Unit, Division of Endoscopy, Copenhagen University Hospital Herlev, Herlev, Denmark;4. Gastroenterology and Liver Services, Bankstown-Lidcombe Hospital, Sydney, Australia;5. Department of Gastroenterology, University Hospitals Leuven, Leuven, Belgium
Abstract:The tendency of particles to aggregate depends on particle-particle and particle-fluid interactions. These interactions can be characterized but it requires accurate 3D measurements of particle distributions. We introduce the application of an off-axis digital holographic microscopy for measuring distributions of dense micrometer (2 μm) particles in a liquid solution. We demonstrate that digital holographic microscopy is capable of recording the instantaneous 3D position of particles in a flow volume. A new reconstruction method that aids identification of particle images was used in this work. About 62% of the expected number of particles within the interrogated flow volume was detected. Based on the 3D position of individual particles, the tendency of particle to aggregate is investigated. Results show that relatively few particles (around 5–10 of a cohort of 1500) were aggregates. This number did not change significantly with time.
Keywords:Three-dimensional microscopy  Three-dimensional image processing  Digital holography  Microscopy  Aggregation
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