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Application of one-step inner-recycling counter-current chromatography for the preparative separation and purification of chemical constituents from the rhizome of Bergenia ciliate (haw.) Sternb |
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| Authors: | Shahid Aziz Faheem Akhter Hidayat Hussain Jiguo Liu Huijiao Yan Li Cui Qixu Chen Wei Cheng Daijie Wang Xiao Wang |
| Affiliation: | 1. Shandong Analysis and Test Center, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China Department of Chemistry, Mirpur University of Science and Technology, Mirpur, Pakistan Biological Engineering Technology Innovation Center of Shandong Province, Shandong Academy of Sciences, Heze Branch of Qilu University of Technology, Heze, China;2. Department of Chemistry, Mirpur University of Science and Technology, Mirpur, Pakistan;3. Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Halle, Germany;4. Heze Peony Development Service Center, Heze, China;5. Shandong Analysis and Test Center, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China;6. Biological Engineering Technology Innovation Center of Shandong Province, Shandong Academy of Sciences, Heze Branch of Qilu University of Technology, Heze, China |
| Abstract: | Bergenia ciliata (haw.) Sternb, the renowned pharmaceutical plant in Jammu and Kashmir of Pakistan, is widely applied in treating different illnesses including diabetes, diarrhea, and vomiting. This work employed an efficient one-step inner-recycling counter-current chromatography for preparative separating and purifying compounds with similar partition coefficients from the rhizome of Bergenia ciliate (haw.). Five compounds, including quercetin rhamnodiglucoside ( 1 ), quercetin-3-O-rutinoside ( 2 ), bergenine ( 3 ), kaempferol ( 4 ), and palmatic acid ( 5 ), were successfully separated using the optimized biphasic solvent system that contained ter-butylmetylether/n-butanol/acetonitrile/water (2:2:1:5, v/v) with the purities over 98%. Mass spectrometry and nuclear magnetic resonance were conducted for structural identification. As a result, our proposed strategy might be applied in separating compounds with similar partition coefficients, which was advantageous with regard to the less solvent and time consumption, and the increased number of theoretical plates. |
| Keywords: | counter-current chromatography one-step inner-recycling mode rhizome of Bergenia ciliate (haw.) sternb separation method similar partition coefficient compounds |