Application of a Cholesterol-Based Stationary Phase for the Analysis of Brevetoxins |
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Authors: | Joseph J. Pesek Maria T. Matyska Tanya Hiltz Jennifer McCall |
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Affiliation: | 1. Department of Chemistry, San Jose State University, San Jose, California, USA;2. MicroSolv Technology Corporation, Leland, North Carolina, USA;3. University of North Carolina Wilmington, College of Health and Human Services, Wilmington, North Carolina, USA |
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Abstract: | A high-performance liquid chromatography protocol for the analysis of brevetoxins has been developed using a silica hydride-based cholesterol column. Brevetoxins are neurotoxins produced by harmful algae that have additional potential as drugs for a number of illnesses/diseases. To develop the optimum conditions, a number of different experimental approaches were tested. These include isocratic and gradient elution, different organic mobile phase components, and temperature variations. A separate protocol was developed for the compounds brevenal and brevenol, also produced by the same algae that make brevetoxins. Brevenal is a natural product under investigation as a therapy for chronic respiratory diseases, such as cystic fibrosis or asthma. The goal of this study was to provide a protocol for the analysis of these compounds that could be further developed into a validated method depending on a particular laboratory's capabilities and to highlight some of the unique features of the cholesterol stationary phase. |
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Keywords: | potential drug silica hydride stationary phase solvent-based selectivity temperature-based selectivity |
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