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Qualitative and quantitative analysis of the chemical components in Yuquan capsules by using ultra-performance liquid chromatography-mass spectrometry |
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| Authors: | Fangfang Wu Zhien Tan Taiping Li Mengli Zhang Yanmei He Shimin Chen Pan Yu Min Ou Lingjie Liu Xijun Wang |
| Affiliation: | 1. National Engineering Laboratory for the Development of Southwestern Endangered, Medicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, P. R. China;2. National Engineering Laboratory for the Development of Southwestern Endangered, Medicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, P. R. China National Chinmedomics Research Center, National TCM Key Laboratory of Serum Pharmacochemistry, Chinmedomics Research Center of State Administration of TCM, Laboratory of Metabolomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Harbin, P. R. China |
| Abstract: | The Yuquan capsules is a commonly used traditional Chinese Patent Medicine used for the treatment of diabetes mellitus. In this study, a high-throughput analytical method for identifying the chemical composition of Yuquan capsules was established for the first time by using ultra-performance liquid chromatography–quadrupole time of flight mass spectrometry. The data obtained were subjected to fragment analysis and this was combined with UNIFI processing of natural products. One-hundred sixteen compounds were characterized from Yuquan capsules. Twelve of the bioactive compounds were quantitatively analyzed by ultra-performance liquid chromatography-tandem triple quadrupole mass spectrometry. This study was undertaken to obtain a comprehensive chemical profile analysis as well as to evaluate the overall quality of Yuquan capsules. The results will provide a reference for the quality evaluation of different Yuquan preparations. In addition, the data will enable basic pharmacodynamic research into these extensively used capsules. |
| Keywords: | qualitative analysis quality control quantitative analysis traditional Chinese medicine Yuquan capsules |