Ultra-high-performance liquid chromatography-Quadrupole-Exactive-Orbitrap-tandem mass spectrometry-based putative identification for Eucommiae Folium (Duzhongye) and its quality-marker candidate for pharmacopeia |
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Authors: | Shuqin Liu Xican Li Rongxin Cai Ban Chen Jingyuan Zeng Chunhou Li Xianxi Zhou Yueying Li |
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Institution: | 1. School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou, P. R. China;2. School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou, P. R. China
School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, P. R. China;3. School of Biological Engineering and Food, Hubei University of Technology, Wuhan, P. R. China;4. School of Basic Medical Science, Guangzhou University of Chinese Medicine, Guangzhou, P. R. China;5. Château de la Source, University Institutes of Technology, University of Orléans, Orléans, France |
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Abstract: | Eucommiae Folium (Duzhongye) is a traditional Chinese medicine with a long history of use in China. However, its quality-marker in Chinese Pharmacopoeia is poorly defined nowadays. The study, therefore, conducted an ultra-high-performance liquid chromatography coupled with hybrid quadrupole-orbitrap tandem mass spectrometry analysis to obtain accurate data. The obtained data were then compared with the authentic standards library using Xcalibur 4.1 software package and TraceFinder General Quan. Through the comparison, the study has putatively identified 26 bioactive compounds, which include 17 flavonoid derivatives (catechin, quercetin 3-gentiobioside, quercetin 3-O-β-D-glucose-7-O-β-D-gentiobioside, taxifolin, myricetin 3-O-galactoside, myricitrin, hyperoside, rutin, isoquercitrin, quercetin 3-O-β-xylopyranoside, quercitrin, isorhamnetin 3-O-β-D-glucoside, quercetin, kaempferol, S-eriodictyol, S-naringenin, and phloridzin), four caffeoylquinic acids (neochlorogenic acid, chlorogenic acid, isochlorogenic acid A, and isochlorogenic acid C), two alkaloids (vincamine and jervine), one lignan (pinoresinol), one xanthone (cowaxanthone B), and one steroid (cholesteryl acetate). Of these, flavonoid isoquercitrin is recommended as the new and additional pharmacopeia quality-marker candidate, which can not only overcome the unreliability of old quality-marker but also recognize the possible counterfeit. |
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Keywords: | counterfeit eucommia ulmoides isoquercitrin pharmacopeia quality-control |
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