| Abstract: | Graphene oxide (GO ), one of the water‐soluble derivatives of graphene, could initiate strong chemiluminescence (CL ) emission of luminol in the absence of any oxidant, and then the CL intensity was inhibited by puerarin (PUE ), a main component in the traditional Chinese medicine Gegen. Based on this, a novel CL method was established for the determination of PUE . This method showed a linear relationship between the CL signal and the logarithm of PUE concentration in the range 0.01–6.00 μM . The limit of detection was 2.83 nM and the relative standard deviation (RSD ) was 1.94% for 11 determinations of 0.4 μM PUE . This method was successfully used for the determination of PUE in Gegen and human urine samples. The possible CL reaction mechanism was investigated by UV –vis, fluorescence, and CL spectra, as well as by some chemical experiments. |
