| Abstract: | Loureirin B (LB), a bioactive drug, is widely used in the treatment of biological diseases. However, due to its poor solution in water, it is important to find the approach which helps LB to specific biological targets. As the most abundant protein in plasma, HSA plays the role of a carrier of numerous drug ligand. Thus, the interaction between LB and HSA was explored by ACE, CE frontal analysis, and pressure‐mediated ACE under simulated physiological conditions (pH 7.4). The binding constants were calculated as 13.14 × 104 L/mol, 7.00 × 104 L/mol, and 2.78 × 104 L/mol for each method, respectively. At the same time, the binding site number (n = 1.429) could be only calculated by the CE frontal analysis method. Furthermore, good experimental repeatability was obtained by pressure‐mediated ACE with RSDs for retention times and peak areas within 2.149 and 1.228, respectively. |
