Supercritical fluid extraction as a tool for isolation of monoterpenes from coniferous needles and walnut-tree leaves |
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| Authors: | Jitka Fojtová Lea Lojková and Vlastimil Kubáň |
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| Institution: | (1) Division of Environmental Science and Ecological Engineering, Korea University, Anam-Dong, Seongbuk-Gu, Seoul, 136-713, Korea;(2) Department of Atmospheric Sciences, Yonsei University, Seoul, 120-749, Korea;(3) Present address: National Park Research Institute, Korea National Park, Namwon-Si, Jeonllabuk-Do, 590-811, Korea; |
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| Abstract: | Several monoterpenes, i.e., (+)-α-pinene, (−)-camphene, sabinene, (−)-β-pinene, myrcene, R-(+)-limonene, (−)-bornylacetate, (−)-trans-caryophyllene and a-humulene were identified and determined by gas chromatography-mass spectrometry (GC-MS) in needles of
Pinaceae (Picea abies, P. omorika, P. pungens, P. Breweriana, Pinus nigra, P. mungo turra, P. black, P. sylvestris, Abies pinsapo, A.
holophylla, A. Bronmuelleris, A. alba, Larix Kaempferi L. decidua) and tree-leaves of Juglandaceae (Juglans regia, J. nigra, J. sieboldiana var. Cordiformis) families. Supercritical fluid extraction (SFE) was found to be very useful for their isolation at optimised conditions (needles/leaves:
pressure 20/30 MPa, temperature 80/130°C, time of extraction 60/60 min, modifier chloroform/chloroform). Their seasonal distribution,
evaluation of differences in concentrations and relative amounts in different trees and their varieties grown in different
localities were evaluated.
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