Repeatability of the measurement of exhaled volatile metabolites using selected ion flow tube mass spectrometry |
| |
Authors: | Piers R Boshier Nandor Marczin and George B Hanna |
| |
Institution: | (1) Departments of Biology and Chemistry, and the Public Health Program, Northern Ontario School of Medicine, Lakehead University, Thunder Bay, Ontario, Canada |
| |
Abstract: | Selected ion flow tube mass spectrometry, SIFT-MS, has been used to determine the repeatability of the analysis of volatile
metabolites within the breath of healthy volunteers, with emphasis on the influence of sampling methodology. Baseline instrument
specific coefficients of variability for examined metabolites were as follows: acetone (1%), ammonia (1%), isoprene (2%),
propanol (6%), ethanol (7%), acetic acid (7%), and hydrogen cyanide (19%). Metabolite concentration and related product ion
count rate were identified as strong determinants of measurement variation. With the exception of ammonia, an orally released
metabolite, variability in repeated on-line breath analysis tended to be lower for metabolites of systemic origin. Standardization
of sampling technique improved the repeatability of the analysis of selected metabolites. Off-line (bag) alveolar breath sampling,
as opposed to mixed (whole) breath sampling, likewise improved the repeatability of the analysis of all metabolites investigated,
with the exception of acetic acid. We conclude that SIFT-MS analysis of common volatile metabolites within the breath of healthy
volunteers is both reliable and repeatable. For selected metabolites, the finding that repeatability is improved through modification
of sampling methodology may have implications in terms of future recommended practices. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|