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Recognition of hairpin DNA from coil DNA by electrospray mass spectrometry with annealing strategy |
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| Authors: | Bo Zhenga b Yi Quan Liua Gu Yuana a Beijing National Laboratory for Molecular Sciences |
| Institution: | Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100086, China ; Shanghai Research Institute of Chemical Industry, Shanghai 200062, China |
| Abstract: | This research presented an annealing strategy to identify hairpin DNA from coil DNA with the same base composition but different arrangements using electrospray mass spectrometry(ESI-MS).A series of single-stranded DNA were annealed with their complementary sequences,respectively.All the five pairs of hairpin DNA and coil DNA were unambiguously distinguished by ESIMS with annealing strategy.This research offers a potential method to probe the DNA structure by comparing with mass spectral characteristics. |
| Keywords: | Hairpin DNA Annealing Mass spectrometry |
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