Double Labeling and Simultaneous Detection of B- and T Cells Using Fluorescent Nano-Crystal (q-dots) in Paraffin-Embedded Tissues |
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Authors: | Eran?Zahavy Esther?Freeman Shlomo?Lustig Avi?Keysary Email author" target="_blank">Shmuel?YitzhakiEmail author |
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Institution: | (1) Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona, 74100, Israel |
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Abstract: | A double immunohistochemical technique for the simultaneous detection of T- and B cells in paraffin-embedded mice tissues
have been developed. This procedure is based on using fluorescent nano-crystals (q-dots). The benefit of using q-dots evolves
from their unique fluorescence characteristics advantages: such as broad excitation spectrum, narrow emission band and high
photo-bleaching threshold compare to organic fluorophores. T cells antigens (CD3) were stained using antibody-coated q-dots
with max emission at 655 nm (GαRb-QD655). B cells antigens (CD45R/B220) were stained using streptavidin-coated q-dots with
max emission at 585 nm (SA-QD585). The simultaneous detection of T- and B cells was demonstrated in paraffin-embedded lymph
node using standard fluorescence microscope. |
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Keywords: | Fluorescence nano-crystals quantum-dots tissues T cells B cells |
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