Liquid chromatographic and mass spectrometric analysis of human serum acid alpha-1-glycoprotein |
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Authors: | Kremmer T Szöllösi E Boldizsár M Vincze B Ludányi K Imre T Schlosser G Vékey K |
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Affiliation: | National Institute of Oncology, Department of Biochemistry, PO Box 21, H 1122 Budapest, Hungary. kremmer@oncol.hu |
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Abstract: | Human serum acid alpha-1-glycoprotein (AGP, orosomucoid) content of healthy individuals and cancer patients was measured, isolated and purified using a protocol of fast and biocompatible sample preparation, ion exchange and dye-ligand affinity chromatographic methods. In comparison to the healthy individuals significantly higher serum AGP levels were found in a wide spectrum of cancer patients, indicating its diagnostic value in the malignant disease. Oligosaccharide content of AGP samples was separated following PNGase F enzyme digestion and analysed by RP-HPLC and MALDI-TOF mass spectrometry. RP-HPLC and MALDI-TOF mass spectrometric analysis of sugar constituents of AGP specimen originated from selected cancer patients with high serum AGP levels indicated the appearance of anomal distribution of bi-, tri- and tetra-antennary oligosaccharide structures compared to the healthy controls. |
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Keywords: | orosomucoid oligosaccharide RP‐HPLC MALDI‐TOF MS |
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