A simple and rapid method for simultaneous determination of benzoic and sorbic acids in food using in-tube solid-phase microextraction coupled with high-performance liquid chromatography |
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Authors: | Yi Wen Ying Wang Yu-Qi Feng |
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Institution: | (1) Department of Chemistry, Wuhan University, Wuhan, 430072, China;(2) Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, 430072, China |
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Abstract: | A simple, rapid and sensitive on-line method for the simultaneous determination of benzoic and sorbic acids in food was developed
by coupling in-tube solid-phase microextraction (SPME) to high-performance liquid chromatography (HPLC) with UV detection.
The diethylamine-modified poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary selected as the extraction
medium exhibited a high extraction capability towards benzoic and sorbic acids. To obtain optimum extraction performance,
several in-tube SPME parameters were investigated, including pH value, inorganic salt, and the organic solvent content of
the sample matrix. After simple dilution with 0.02 mol/L phosphate solution (pH 4.0), carbonated drink, juice drink, sauce
and jam samples could be directly injected for extraction. For succade samples, a small amount of acetonitrile was required
to extract analytes prior to dilution and subsequent extraction. The linearity of the method was investigated over a concentration
range of 5–20000 ng/mL for both analytes, and the correlation coefficients (R
2 values) were higher than 0.999. The detection limits for benzoic and sorbic acids were 1.2 and 0.9 ng/mL, respectively. The
method reproducibility was tested by evaluating the intra- and interday precisions; relative standard deviations of less than
4.4 and 9.9%, respectively, were obtained. Recoveries of compounds from spiked food samples ranged from 84.4 to 106%. The
developed method was shown to be suitable for the routine monitoring of benzoic and sorbic acids in various types of food
samples. |
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Keywords: | Benzoic and sorbic acids Food In-tube solid-phase microextraction High-performance liquid chromatography Diethylamine-modified poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary |
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