| 卟啉全内反射同步荧光法测定蛋白质 |
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| 引用本文： | 陈莹,姚闽娜,唐尧基,李耀群. 卟啉全内反射同步荧光法测定蛋白质[J]. 光谱学与光谱分析, 2005, 25(12): 2048-2051 |
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| 作者姓名： | 陈莹 姚闽娜 唐尧基 李耀群 |
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| 作者单位： | 厦门大学化学化工学院与现代分析科学教育部重点实验室，福建 厦门 361005 |
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| 基金项目： | 教育部优秀青年教师资助计划项目（教人司[2001]39号）和国家自然科学基金（29875023）资助项目 |
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| 摘 要： | 应用全内反射与同步荧光相结合的技术，建立了利用蛋白质吸附在液-固界面上的同步荧光信号来测定本体蛋白质溶液浓度的新方法。其原理为meso-四(4-磺酸基苯基)卟啉(TPPS)标记牛血清蛋白(BSA)在石英界面的吸附后信号强度随本体溶液浓度的增加呈线性关系。方法的检出限是94 ng·mL-1。用于实际人血清蛋白样品的测定，结果令人满意。
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| 关 键 词： | 全内反射 同步荧光 卟啉 血清蛋白 |
| 文章编号： | 1000-0593(2005)12-2048-04 |
| 收稿时间： | 2004-06-28 |
| 修稿时间： | 2004-10-08 |
Determination of Protein with TPPS by Total Internal Reflection Synchronous Fluorescence Spectroscopy |
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| CHEN Ying,YAO Min-na,TANG Yao-ji,LI Yao-qun. Determination of Protein with TPPS by Total Internal Reflection Synchronous Fluorescence Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2005, 25(12): 2048-2051 |
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| Authors: | CHEN Ying YAO Min-na TANG Yao-ji LI Yao-qun |
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| Affiliation: | Department of Chemistry, the MOE Key Laboratory of Analytical Sciences, Xiamen University, Xiamen 361005, China |
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| Abstract: | A new method of quantitative determination for serum albumin in aqueous solution has been developed by measuring total internal reflection synchronous fluorescence at the solid/liquid interface. The combination of bovine serum album in (BSA) and meso-tetrakis (4-sulphonatophenyl) porphyrin (TPPS) adsorbed onto the glass surface produced a synchronous fluorescence signal at 421 nm. At pH 4.25, the signal intensity of BSA adsorbed on the interface was proportional to the BSA concentration in bulk solution. The linear range of 1.0-8.0 μg·mL-1 and the detection limit of 0.94 μg·mL-1 were obtained. The human serum samples were determined with satisfactory results. |
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| Keywords: | Total internal reflection Synchronous fluorescence TPPS Protein |
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