A single reagent radioimmunoassay for thyroxine in blood samples absorbed on filter paper for mass screening of neonatal hypothyroidism |
| |
| Authors: | N. Nair M. R. A. Pillai R. S. Mani S. Naik M. Desai P. Upadhye M. P. Colaco |
| |
| Affiliation: | 1. Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Trombay, 400 085, Bombay, (India)
2. B. J. Wadia Hospital for Children and Institute of Child Health, Parel, 400 012, Bombay, (India)
|
| |
| Abstract: | A single reagent radioimmunoassay for thyroxine in blood samples absorbed on filter paper for the mass screening of neonatal hypothyroidism is described. Blood samples were collected by pricking the heel of newborn babies (3 days old) and pressing Whatman 3 filter paper against the wound. 6 mm diameter blood spots were punched out at the time of assay and incubated with 0.4 ml of a preincubated antigen-antibody complex for six hours at 37 °C. 1 ml of 22% polyethylene glycol is used for the precipitation of antigen-antibody complex. The assay has a sensitivity of 2.2 ng/ml. 500 samples collected from newborns were analyzed in the assay and gave a mean of 117.6±31.9 ng/ml. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
