Anwendung einer S?ule zur Extraktion, Trocknung und Reinigung von Retinol und α-Tocopherol bei deren Bestimmung in Milch und Milchprodukten mit Hilfe der HPLC

Summary An already described HPLC method for the determination of retinol and -tocopherol has been improved by development of the simple column serving simultaneously for the extraction, drying and purification of these compounds. This column contains alumina, extrelut and sodium sulphate. After saponification of the sample the solution is neutralized and applied to the column. Hexane is employed for extracting the vitamins. Preparation of the column and of a suitable rack are described in detail.