Combined dielectrophoretic field cages and laser tweezers for electrorotation |
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| Authors: | T Schnelle T Müller C Reichle G Fuhr |
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| Institution: | Humboldt-Universit?t zu Berlin, Institut für Biologie, Invalidenstr. 42, 10115 Berlin, Germany (Fax: +49-030/290-38635, E-mail: fuhr@rz.hu-berlin.de), DE
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| Abstract: | Symmetric rotating octode field cages do not allow simultaneous particle trapping and electrorotation. Laser-trapped particles
can stably be held in inhomogeneous rotating electric fields. Therefore, in a combination of laser tweezers and field-cage
(electro-optical cage) dielectric single-particle spectroscopy (DSPS) of particles and cells can be carried out at low electric
field strength. The electro-optical cage overcomes the limitation of low enough effective particle conductivity ensuring repelling
dielectrophoretic forces in conventional field cages. At low power, forces in field cages and laser tweezers hardly interfere
and can be used to calibrate each other.
Received: 3 May 1999 / Revised version: 16 August 1999 / Published online: 27 October 1999 |
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| Keywords: | PACS: 41 20 Bt 42 62-b 42 62 Be 47 32 -y 61 20 Ja 87 80 s +94 30 Hn |
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