高效液相色谱法同时测定调味料中的8种合成着色剂

建立一种同时测定调味料中8种合成着色剂的高效液相色谱分析方法。样品经30％甲醇水溶液提取，采用Diamonsil—C18柱（250mm×4．6mm），以甲醇-1．55g／L乙酸铵为流动相，在最佳梯度洗脱条件下对8种合成着色剂进行分离，二极管阵列检测器检测，外标法定量。8种合成着色剂的线性范围为0．5～10．0μg／mL，回收率为95．0％～101％。对样品进行6次重复测定，峰面积测定结果的相对标准偏差为0．84％-3．51％（n=6）。

Simultaneous Determination of 8 Synthetic Colours in Condiment by High Performance Liquid Chromatography

A high performance liquid chromatographic method was developed for the simr~ltaneous determination of 8 synthetic eolours in condiment. The sample was extracted with methanol water solution, separated on Diamonsil- C18（250 mm× 4.6 mm） column with methanol-l.55 g / L ammonium acetate as mobile phase by gradient elution. The target compounds were confirmed by diode-array detection and quantified by the external standnrd method. The results showed that the linear range of 8 synthetic colours was 0.5-10.0 μ g/mL and recovery was 95.0%-101.0%. The relative standard deviation of six times repeated measurements of the peak area was 0. 84%-3.51%（n=6）.