| 食用植物油中胆固醇含量的快速测定 |
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| 引用本文： | 廖连英,韩飞,赵质创,施煜,胡宏灿,林悦凯,石声鑫,卢仁杰. 食用植物油中胆固醇含量的快速测定[J]. 化学分析计量, 2012, 21(2): 32-33 |
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| 作者姓名： | 廖连英 韩飞 赵质创 施煜 胡宏灿 林悦凯 石声鑫 卢仁杰 |
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| 作者单位： | 广东省汕头市质量计量监督检验所,广东省质量监督食品检验站(汕头),广东汕头515041 |
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| 基金项目： | 广东省质量监督食品检验站(汕头)食品类风险监测专项 |
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| 摘 要： | 将食用植物油直接用无水乙醇完全溶解，经滤膜过滤后，用高效液相色谱法测定其中的胆固醇含量。HPLC条件：Diamonsil C18色谱柱（250mm×4．6mm，5μm），100％甲醇为流动相，流速1．0mL／min，柱温38℃，检测波长208nm。方法的检出限为50mg／L，加标回收率在91．5％-101．4％之间，测定结果的相对标准偏差为2．12％-4．15％。该法适用于食用植物油中胆固醇含量的快速测定。
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| 关 键 词： | 胆固醇 食用植物油 高效液相色谱 |
Rapid Determination of Cholesterol in Vegetable Oil |
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| Liao Lianying , Han Fei , Zhao Zhichuang , Shi Yu , Hu Hongcan , Lin Yuekai , Shi Shengxin , Lu Renjie. Rapid Determination of Cholesterol in Vegetable Oil[J]. Chemical Analysis And Meterage, 2012, 21(2): 32-33 |
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| Authors: | Liao Lianying Han Fei Zhao Zhichuang Shi Yu Hu Hongcan Lin Yuekai Shi Shengxin Lu Renjie |
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| Affiliation: | [Guangdong Shantou Institute of Quality and Metrology Supervision Testing,Guangdong Food Supervision Inspection Station(Shantou),Shantou 515041,China] |
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| Abstract: | Samples of vegetable oil were treated with ethanol and filtered with membrane, which were used for the determination of cholesterol content by HPLC. HPLC conditions： Diamonsil C18 column （250 mm × 4.6 mm, 5 μ m） was used, the mobile phase was 100% acetonitrile with flow rate of 1.0 mL/min, Column temperature was 38 ℃, UV-detector was carried out at the wavelength of 208 nm. Detection limit of the method was 50 mg/L, the recovery was in the range of 91.5% - 101.4%, and the relative standard deviation was 2.12% - 4.15%. The method was suitable for rapid determination of cholesterol in vegetable oil. |
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| Keywords: | cholesterol vegetable oil high performance liquid chromatography |
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