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Application of 4-methyl-7-methoxycoumarin derivatives to the high pressure liquid chromatographic analysis of fatty acids
Authors:S. G. Zelenski  J. W. Huber III
Affiliation:(1) School of Pharmacy, Northeast Louisiana University, 71209 Monroe, Louisiana, USA
Abstract:Summary A high pressure liquid chromatographic method of fatty acid analysis has been developed using the fluorescent label, 4-bromomethyl-7-methoxycoumarin. Two simplified procedures for derivatization using sealed reaction vessels allow derivatization of volumes of 200 to 500 mm3 and 2 to 5 mm3 respectively.
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