Voltammetric determination of dipeptidyl aminopeptidases I and IV

Dipeptidyl aminopeptidases I and IV have recently found wide practical application (polypeptide sequencing, clinical diagnostics) so it is necessary to extend present methods and work out new ones for determination of their activity. A new, voltammetric method for determination of peptidase activity is proposed, which allows optimization of the determination conditions according to the activity to be measured. The method has some advantages over the spectrophotometric methods, such as applicability to opaque solutions and higher sensitivity.