Reversible immobilization of engineered molecules by Ni-NTA chelators |
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Authors: | Maly J Di Meo C De Francesco M Masci A Masojidek J Sugiura M Volpe A Pilloton R |
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Affiliation: | Department of Biology, University of J.E. Purkyne, Usti nad Labem, Czech Republic. |
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Abstract: | Electrochemical synthesis of nickel-nitrilotriacetic acid (Ni-NTA) chelators, for subsequent immobilization of (His)(6)-tagged proteins (Photosystem II (PSII) as model molecule), on Au or Au-graphite electrodes is compared to chemical synthesis. Results show: (i) higher Ni-NTA surface density, (ii) shorter treatment time (1-12 min vs. 16 h normally needed for self-assembled monolayer (SAM)), (iii) possibility of addressing the chelator to only one Au electrode, in a sensor micro-array. |
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