Effect of surface attachment on synthesis of bacterial cellulose |
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Authors: | Barbara R Evans Hugh M O’Neill |
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Institution: | (1) Chemical Sciences Division, Oak Ridge National Laboratory, 4500N Bethel Valley Road, 37831-6194 Oak Ridge, TN;(2) Department of Biochemistry & Molecular & Cellular Biology, University of Tennessee, 37996-0840 Knoxville, TN |
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Abstract: | Gluconacetobacter spp. synthesize a pure form of hydrophilic cellulose that has several industrial specialty applications. Literature reports
have concentrated on intensive investigation of static and agitated culture in liquid media containing high nutrient concentrations
optimized for maximal cellulose production rates. The behavior of these bacteria on semisolid and solid surfaces has not been
specifically addressed. The species Gluconacetobacter hansenii was examined for cellulose synthesis and colony morphology on a range of solid supports, including cotton linters, and on
media thickened with agar, methyl cellulose, or gellan. The concentration and chemical structure of the thickening agent were
found to be directly related to the formation of contiguous cellulose pellicules. Viability of the bacteria following freezer
storage was improved when the bacteria were frozen in their cellulose pellicules.
This article was authored by a contractor of the US government under contract no. DEAC05-00OR22725. Accordingly, the US government
retains a nonexclusive, royalty-free license to publish or reproduce the published form of this contribution, or allow others
to do so, for US government purposes. |
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Keywords: | Gluconacetobacter hansenii cellulose pellicule gellan agar |
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