Determination of 6-mercaptopurine and its metabolites in plasma or serum by high performance liquid chromatography |
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| Authors: | Z Sahnoun F Serre-Debeauvais J Lang G Faucon M Gavend |
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| Institution: | Laboratoire de Pharmacologie Clinique, H?pital Edouard Herriot, Lyon, France. |
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| Abstract: | A sensitive and accurate reversed phase liquid chromatographic assay was developed for the determination of 6-mercaptopurine (6MP) (the active metabolite of azathioprine) in human plasma. The assay involved extraction into acetonitrile and dichloromethane from plasma pretreated with 0.038 M of dithiothreitol solution. The residue was analyzed by isocratic chromatography on a C18 analytical column with UV detection at 326 nm. The average extraction recovery of 6MP was 85%. The method has been applied successfully to the determination of 6MP and its metabolites in pharmacokinetic studies. |
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