| Increase of Calcium Levels at Interphase in NIH 3T3 Cells |
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| 引用本文： | 薛绍白,张鸿卿,成汝萱,李素文. Increase of Calcium Levels at Interphase in NIH 3T3 Cells[J]. 中国科学B辑(英文版), 1993, 0(3) |
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| 作者姓名： | 薛绍白 张鸿卿 成汝萱 李素文 |
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| 作者单位： | Department of Biology，Belling Normal University，Beijing 100875，PRC，Department of Biology，Belling Normal University，Beijing 100875，PRC，Department of Biology，Belling Normal University，Beijing 100875，PRC，Department of Biology，Belling Normal University，Beijing 100875，PRC |
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| 基金项目： | Project supported by NSFC and DFEC. |
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| 摘 要： | The fluorescent calcium ion indicator dye Fluo-3 and DNA-binding dye Hoechst 33342 were employed to determine, in a quantitative microspectrofluorometric study, the intracellular calcium ion concentration ([Ca~(2+)]_i) and the DNA content of individual living NIH3T3 cells. The well-separated excitation and emission properties of these dyes allowed us to establish for each cell both the phase of the cell cycle using DNA content and [Ca~(2+)]_i. We found that the transition from G_1, through S, to the G_2 phase is accompanied by a two-fold increase in [Ca~(2+)]_i. The [Ca~(2+)]_i was inhomologous in each phase of the interphase (G_1, S and G_2) although [Ca~(2+)]_i in the S and G_2 phases was never lower than certain threshold values in the G_1 and S phases respectively. [Ca~(2+)]_i in G_0 cells was lower than that in G_2 cells. These changes in [Ca~(2+)]_i suggest that [Ca~(2+)]_i may be an import regulator of cell cycle progression.
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Increase of Calcium Levels at Interphase in NIH 3T3 Cells |
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| XUE Shao-Bai ZHANG Hong-Qing CHENG Ru-Xuan and LI Su-Wen. Increase of Calcium Levels at Interphase in NIH 3T3 Cells[J]. Science in China(Chemistry), 1993, 0(3) |
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| Authors: | XUE Shao-Bai ZHANG Hong-Qing CHENG Ru-Xuan and LI Su-Wen |
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| Abstract: | The fluorescent calcium ion indicator dye Fluo-3 and DNA-binding dye Hoechst 33342 were employed to determine, in a quantitative microspectrofluorometric study, the intracellular calcium ion concentration ([Ca~(2+)]_i) and the DNA content of individual living NIH3T3 cells. The well-separated excitation and emission properties of these dyes allowed us to establish for each cell both the phase of the cell cycle using DNA content and [Ca~(2+)]_i. We found that the transition from G_1, through S, to the G_2 phase is accompanied by a two-fold increase in [Ca~(2+)]_i. The [Ca~(2+)]_i was inhomologous in each phase of the interphase (G_1, S and G_2) although [Ca~(2+)]_i in the S and G_2 phases was never lower than certain threshold values in the G_1 and S phases respectively. [Ca~(2+)]_i in G_0 cells was lower than that in G_2 cells. These changes in [Ca~(2+)]_i suggest that [Ca~(2+)]_i may be an import regulator of cell cycle progression. |
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| Keywords: | intracellular calcium fluo-3 cell cycle Go cell p34~(cdex). |
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