Influence of mixing regime on enzymatic saccharification of steam-exploded softwood chips |
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Authors: | Mais Ursula Esteghlalian Ali R Saddler John N |
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Institution: | (1) Forest Products Biotechnology, Faculty of Forestry, The University of British Columbia, 4033-2424 Main Mall, Forest Sciences Centre, V6T 1Z4 Vancouver, BC, Canada |
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Abstract: | In an attempt to elucidate the effect of reduced mixing on the enzymatic hydrolysis of lignocellulosic feedstocks, a pretreated
softwood substrate was hydrolyzed under various mixing regimes using a commercial cellulase mixture. The substrate was generated
by SO2-catalyzed steam explosion of Douglas fir wood chips followed by alkali-peroxide treatment to remove lignin. Three mixing
regimes were tested; continuous mixing at low (25 rpm) and high (150 rpm) speeds, and mixing at low-speed interspersed with
5-min intervals of high-speed agitation at 150 rpm. At both substrate concentrations (7.5 and 10% w/w]), the mixed-speed
mixing was able to produce sufficiently high conversion rates and yields (93% after 96 h), close or slightly better than those
obtained under vigorous mixing (150 rpm). The low-speed shaking produced appreciably lower conversion yields at both levels
of substrate concentration. Therefore, the mixed-speed regime may be a viable process option, because it does not seem to
have an adverse impact on the cellulose conversion yield and can be an effective means of reducing the mixing energy requirements
of an enzymatic hydrolysis process. |
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Keywords: | Enzymatic hydrolysis mixing softwoods Douglas fir bioconversion |
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