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Experimental and numerical study on simultaneous effects of scattering and absorption on fluorescence spectroscopy of a breast phantom
Authors:Mohammad Ali Ansari  Reza Massudi  Marjaneh Hejazi
Institution:1. Laser and Plasma Research Institute, Shahid Beheshti University, Evin, Tehran, Iran;2. Department of Medical Physics and Biomedical Systems, Faculty of Medicine, Tehran University of Medical Sciences, and Research Centre for Science and Technology in Medicine (RCSTIM), Tehran, Iran;1. College of Sciences, Huazhong Agricultural University, 430070 Wuhan, PR China;2. Optical and Electronic Information, Huazhong University of Science and Technology, 430070 Wuhan, PR China;1. Department of Mechanical Systems Engineering, Asahikawa National College of Technology, 2-2-1-6 Syunkodai, Asahikawa, Hokkaido 071-8142, Japan;2. Department of Mechanical Engineering, Kushiro National College of Technology, 2-32-1 Otanoshike-nishi, Kushiro, Hokkaido 084-0916, Japan;3. Biomedical Engineering Department, Tokyo City University, 1-28-1 Tamazutsumi, Setagaya, Tokyo 158-8557, Japan;4. Division of Engineering for Composite Functions, Muroran Institute of Technology, 27-1 Mizumoto-cho, Muroran, Hokkaido 050-8585, Japan;1. Department of Mechanical Systems Engineering, Asahikawa National College of Technology, 2-2-1-6 Syunkodai, Asahikawa, Hokkaido 071-8142, Japan;2. College of Design and Manufacturing Technology, Muroran Institute of Technology, 27-1 Mizumoto-cho, Muroran, Hokkaido 050-8585, Japan
Abstract:Detection of dysplastic lesions can decrease morbidity and mortality caused by cancer. The fluorescence spectroscopy is a noninvasive method of detecting dysplasia in several organs. During dysplastic progression, fluorescence intensity of spectrum is changed due to variation in absorption and scattering coefficients of tissue. In this work we have experimentally verified simultaneous effects of scattering and absorption coefficients on fluorescence intensity of different tissue like phantoms with the same optical properties as the human breast ductal carcinoma. The results are compared with those obtained by Monte Carlo simulation and good agreement between them is observed. This provides an important detecting method to discriminate dysplastic tissue from normal tissue.
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