Development of FIA equipped with a chemiluminescence detector using a mixed reagent of luminol and 1,10-phenanthroline. |
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Authors: | Kazuhiko Tsukagoshi Masayuki Tahira Riichiro Nakajima |
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Institution: | Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan. |
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Abstract: | We developed an FIA system equipped with a chemiluminescence detector using a mixed chemiluminescence reagent of luminol and 1,10-phenanthroline for the detection of metal ions and metal complexes. The carrier, mixed chemiluminescence reagent comprising luminol, 1,10-phenanthroline, and cethyltrimethylammonium bromide, and H2O2 solutions were fed by corresponding pumps at a definite flow rate. Sample solutions dissolving hematin, Co(NH3)4(H2O)2]2(SO4)3, CuSO4, NiCl2, K3Fe(CN)6], and K4Fe(CN)6] were analyzed as models by the means of the present FIA system. Solutions of hematin, Co(NH3)4(H2O)2]2(SO4)3, CuSO4, and NiCl2 were detected as positive peaks, as usual. The order of the catalytic activity of these samples for the present chemiluminescence reaction using the mixed chemiluminescence reagent was Co(NH3)4(H2O)2]2(SO4)3 > hematin > CuSO4 > NiCl2. On the other hand, sample solutions of K3Fe(CN)6] and K4Fe(CN)6] were detected as negative peaks and were determined over the ranges of 1 x 10(-8) - 1 x 10(-6) M with a detection limit of 1 x 10(-8) M and 2 x 10(-8) - 4 x 10(-6) M with a detection limit of 2 x 10(-8) M, respectively. Their negative peaks were observed reproducibly with a relative standard deviation of 2 - 5%. |
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