Magnesium ion selective two-photon fluorescent probe based on a benzo[h]chromene derivative for in vivo imaging |
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| Authors: | Kim Hwan Myung Yang Pil Rye Seo Mun Sik Yi Jae-Sung Hong Jin Hee Jeon Seung-Joon Ko Young-Gyu Lee Kyoung J Cho Bong Rae |
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| Institution: | Molecular Opto-Electronics Laboratory, Department of Chemistry and Center for Electro- and Photo-Responsive Molecules, Graduate School of Life Sciences and Biotechnology, Korea University, 1-Anamdong, Seoul 136-701, Korea. |
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| Abstract: | A novel, two-photon probe for the detection of free Mg2+ ions in living cells and live tissues has been developed. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescence in response to Mg2+ ions, can be easily loaded into the cell and tissue, shows high photostability, and can measure the Mg2+ ion concentration without interference by Ca2+ ions in living cells. The intracellular dissociation constant (Kdi) for Mg2+ determined by the two-photon process is 2.5 mM, which is suitable for dynamic Mg2+ concentration measurement. In addition, the probe is capable of imaging endogenous stores of free Mg2+ at a few hundred micrometers depth in live tissues using two-photon microscopy (TPM). |
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