Anion-lnduced Fluorescence Quenching of a New Zwitterionic Biacridine Derivative |
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Authors: | Tobias Werner Karsten Fahnrich Christian Huber Otto S Wolfbeis |
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Institution: | University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, Regensburg, Germany |
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Abstract: | The effect of halides and different buffer anions on the quenching of the fluorescence of the new probe 10,10′-bis(3-sulfopropyl)-9,9′-biacridine (SPBA) has been studied using fluorescence and decay time measurements. The linearity of the Stern-Volmer plot indicates that fluorescence quenching by halides can be described reasonably well by a single-exponential decay with a K of 4.06 times 106M-1s-1for chloride, 7.83 times 106M-1s-1for bromide and 1.12 times 107M-1s-1for iodide. We have found that SPBA is collisionally quenched also by the buffers 3-(N-mor-pholino)propanesulfonic acid (MOPS) and N-2-hydroxy-ethylpiperazine-N′-ethansulfonic acid (HEPES). The bi-molecular rate constants are 1.67 × 106M-ls-1for HEPES and 1.44 times 106M-1s-1for MOPS. |
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