Methodology description for detection of cellular uptake of PVA coated superparamagnetic iron oxide nanoparticles (SPION) in synovial cells of sheep |
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| Affiliation: | 1. Musculoskeletal Research Unit, Equine Hospital, Vetsuisse Faculty Zurich, University of Zurich, Winterthurerstr. 260, 8057 Zurich, Switzerland;2. Powder Technology Laboratory, Institute of Materials, Swiss Federal Institute of Technology Lausanne, EPFL, MX-D Ecublens, 1015 Lausanne, Switzerland;3. MatSearch, Ch. Jean Pavillard 14, 1009 Pully, Switzerland;1. Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, British Columbia, Canada;2. Biomedical Engineering Department, The Cleveland Clinic Foundation, Cleveland, Ohio, USA;1. Department of Materials Chemistry, Nanchang Hangkong University, Nanchang 330063, PR China;2. College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China;3. Department of Applied Chemistry, East China Institute of Technology, Fuzhou 344000, PR China;4. Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, PR China;1. Universidade Estadual Paulista (UNESP), Departamento de Parasitologia, Instituto de Biociências; Botucatu - SP, Brazil;2. University of Calgary, Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine; Calgary, Alberta, Canada;1. Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran;2. Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran;3. Department of Radiation Oncology, Milad Hospital, Isfahan, Iran;4. Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran |
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| Abstract: | The detection of superparamagnetic iron oxide nanoparticles (SPION) in synoviocytes is reported. Synoviocytes were incubated for 2, 12, 24 and 48 h with 1.5 mg/ml of PVA coated SPION under the influence of magnets (12 h). Particles were well tolerated by the synoviocytes, were easily detected using the Turnbulls and Prussian blue reactions between 12 and 24 h. |
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