江浙蝮蛇蛇毒蛋白C激活因子基因的克隆及测序

从江浙蝮蛇毒腺中抽提总RNA，RT－PCR进行体外扩增，获得江浙蝮蛇蛇毒蛋白C激活因子基因，克隆至pGEX-5X-3载体中，对3个重组克隆分别作DNA全序列分析，通过遗传密码推导出相应的氨基酸序列，与其它已知的丝氨酸复白酶蛇毒蛋白的氨基酸作比较，其中许多上氨基酸有很强的同源性，该基因的成功克隆，不仅推导出江浙蝮蛇蛇毒蛋白C激活因子的蛋白质序列，也为进一步开展江浙蝮蛇蛇毒蛋白C激活因子蛋白质工程的研究工作打下良好的基础。

Cloning and sequencing of protein C activator gene from Agkistrodon blomhoffii brevicaudus

Total RNA was extracted from the gland of the snake Agkistrodon blomhoffii hrevicaudus. The protein C activator gene then was amplified by RT-PCR and cloned into the pGEX-5x-3 vector. Its nucleotide sequence was determined by analyzing the full DNA sequence of three colonies containing the protein C activator gene. The deduced gene amino acid sequence is analogous to other serine proteases from snakes. The successful cloning of the protein C activator gene not only made the determination of its total amino acid sequence possible, but also provided a good basis for further research work in the protein engineering of protein C activator from snakes.