Exploration of Potential Tumor Markers for Lung Adenocarcinomas by Two‐Dimensional Gel Electrophoresis Coupled with Nano‐LC/MS/MS |
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| Authors: | Horng‐Jyh Harn Yi‐Lin Chen Po‐Cheng Lin Yeung‐Leung Cheng Shin‐Chun Lee Tzyy‐Wen Chiou Hsueh‐Hui Yang |
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| Institution: | 1. Pathology Department, China Medical University & Hospital, Taichung 40402, Taiwan, R.O.C.;2. Graduate Institute of Biotechnology, National Ilan University, Ilan 26047, Taiwan, R.O.C.;3. Graduate Institute of Biotechnology, National Dong Hwa University, Hualien 97002, Taiwan, R.O.C.;4. Division of Thoracic Surgery, Department of Surgery, Tri‐Service General Hospital, Taipei 11490, Taiwan, R.O.C.;5. Laboratory of Herbal Medicine and Molecular Oncology in Division of Thoracic Surgery, Department of Surgery, Tri‐Service General Hospital, National Defense Medical Center, Taipei 11490, Taiwan, R.O.C.;6. Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien 97002, Taiwan, R.O.C.;7. General Education Center, Tzu Chi College of Technology, Hualien 97002, Taiwan, R.O.C. |
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| Abstract: | To explore the potential tumor markers for lung adenocarcinoma, two‐dimensional gel electrophoresis (2DE) coupled with nano‐LC/MS/MS was used to analyze the differentially expressed proteins in 10 surgical resected lung adenocarcinoma tissues. 16 proteins were significantly different between the cancer tissue and adjacent normal tissue. Galectin‐1, peroxiredoxin II (Prx II), proapolipoprotein, glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), aldolase A, enolase 1, neuropolypeptide h3, Prx V, cyclophilin A, vimentin, protein disulfide isomerase (PDI), tropomyosin 3 (TPM 3), glutathione S‐transferase Pi (GST‐Pi), manganese superoxide dismutase (MnSOD), and cofilin 1 were up‐regulated in the cancer tissue. On the other hand, profilin was down‐regulated in the cancer tissue. Among these proteins, six proteins were validated by Western blot analysis. The identified proteins contributing to the spectrum of cancer progression may be used as potential diagnostic biomarkers for lung adenocarcinomas. |
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| Keywords: | Nanoscale capillary LC/MS/MS Proteomics Lung adenocarcinomas |
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