| 综述(187~191)利用冷冻聚焦离子束制备冷冻含水切片技术问题探讨 |
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| 引用本文： | 贾星.综述(187~191)利用冷冻聚焦离子束制备冷冻含水切片技术问题探讨[J].分析测试技术与仪器,2020,26(3):187-191. |
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| 作者姓名： | 贾星 |
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| 作者单位： | 中国科学院 生物物理研究所, 北京 100101 |
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| 摘 要： | 冷冻聚焦离子束制备冷冻含水切片为研究细胞内原位结构打开了窗口, 其切片质量直接影响了后续冷冻电子断层扫描成像数据的收集及处理.目前, 这一技术的应用还存在各种各样的问题, 总结了近期文献中的相关解决之道, 以期为制备冷冻含水切片的研究者提供参考.
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| 关 键 词： | 冷冻聚焦离子束 冷冻含水切片 细胞 自动切割 |
| 收稿时间： | 2020-07-07 |
Discussion on Technical Problems Using Cryo-Focused Ion Beam for Cryo-Lamellae Preparation |
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| Institution: | Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | The use of Cryo-focused ion beam milling (Cryo-FIB) to prepare the cryo-lamellae opens a window for the study of in situ cellular structure. The quality of the lamellae will directly affect the data collection and processing of cryo-electron tomography. Since there are still different problems in the application of the technology, we summarized some relative solutions published in recent papers, in order to provide references for researchers doing Cryo-FIB experiments. |
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